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pcdna3 trif cfp  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 trif cfp
    Pcdna3 Trif Cfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+trif+cfp/pcDNA3-TRIF-CFP+(Plasmid+%2313644)/bio_rxiv__64898__2026__01__23__701331-157-59-66
    Average 93 stars, based on 11 article reviews
    pcdna3 trif cfp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: Interference of host innate immune response by hepatitis E virus
    Article Snippet: .. The pCDNA3 -TRIF-CFP (82) and pRK5-HA- Ubiquitin-K63 (209) were obtained from Addgene. ..

    Plasmid Preparation:

    Article Title: Toll-like Receptor (TLR)-induced Rasgef1b expression in macrophages is regulated by NF-κB through its proximal promoter.
    Article Snippet: Ras Guanine Exchange Factor (RasGEF) domain family member 1b is encoded by a Toll-like receptor (TLR)inducible gene expressed in macrophages, but transcriptional mechanisms that govern its expression are still unknown.. Here, we have functionally characterized the 5 flanking Rasgef1b sequence and analyzed its transcriptional activation.. We have identified that the inflammation-responsive promoter is contained within a short sequence (-183 to +119) surrounding the transcriptional start site.

    Article Title: TAK1 integrates the NLRP1 inflammasome into the innate immune response to double-stranded RNA
    Article Snippet: .. RIG-I and MDA5 were subcloned into the pQ-PGK-XIP backbone using SbfI and NotI restriction sites. pEFTak-FLAG-MAVS was a gift from Dr. Nandan Gokhale, and MAVS was subcloned into the pQ-PGK-XIP backbone using SbfI and NotI sites. hTLR3-pcDNA3 was a gift from Saumen Sarkar (Addgene plasmid # 32712) and TLR3 was subcloned into pQ-PGK-XIP using SbfI and NotI restriction sites. pcDNA3-TRIF-CFP was a gift from Doug Golenbock (Addgene plasmid # 13644) and was cloned into pQ-PGK-XIP using InFusion HD (Clonetech) pDONR223-MAP3K7 was a gift from William Hahn & David Root (Addgene plasmid # 23693) and MAP3K7 was subcloned into pQ-PGK-XIP using SbfI and NotI restriction sites. .. Point mutations in MAP3K7 were introduced using overlapping extension PCR. pcDNA4/TO/Strep-HA-ZAK alpha was a gift from Simon Bekker-Jensen (Addgene plasmid # 141193), and ZAKα was subcloned into pQ-PGK-XIP using SbfI and NotI restriction sites. pLKO-puro-CARD8 generated previously .

    Transfection:

    Article Title: Anesthetic agent propofol inhibits myeloid differentiation factor 88-dependent and independent signaling and mitigates lipopolysaccharide-mediated reactive oxygen species production in human neutrophils in vitro.
    Article Snippet: Engagement of toll-like receptor 4 (TLR4) can activate the myeloid differentiation factor 88 (MyD88)/tollinterleukin-1-resistance domain-containing adapter-inducing interferon-β (TRIF) dependent pathways, inducing production of reactive oxygen species (ROS) in neutrophils.. Propofol (PPF) has both antioxidant and anti-inflammatory properties.. However, the molecular mechanism by which PPF influences human neutrophil function is yet to be elucidated.

    Control:

    Article Title: Anesthetic agent propofol inhibits myeloid differentiation factor 88-dependent and independent signaling and mitigates lipopolysaccharide-mediated reactive oxygen species production in human neutrophils in vitro.
    Article Snippet: Engagement of toll-like receptor 4 (TLR4) can activate the myeloid differentiation factor 88 (MyD88)/tollinterleukin-1-resistance domain-containing adapter-inducing interferon-β (TRIF) dependent pathways, inducing production of reactive oxygen species (ROS) in neutrophils.. Propofol (PPF) has both antioxidant and anti-inflammatory properties.. However, the molecular mechanism by which PPF influences human neutrophil function is yet to be elucidated.

    Electroporation:

    Article Title: Anesthetic agent propofol inhibits myeloid differentiation factor 88-dependent and independent signaling and mitigates lipopolysaccharide-mediated reactive oxygen species production in human neutrophils in vitro.
    Article Snippet: Engagement of toll-like receptor 4 (TLR4) can activate the myeloid differentiation factor 88 (MyD88)/tollinterleukin-1-resistance domain-containing adapter-inducing interferon-β (TRIF) dependent pathways, inducing production of reactive oxygen species (ROS) in neutrophils.. Propofol (PPF) has both antioxidant and anti-inflammatory properties.. However, the molecular mechanism by which PPF influences human neutrophil function is yet to be elucidated.

    Clone Assay:

    Article Title: TAK1 integrates the NLRP1 inflammasome into the innate immune response to double-stranded RNA
    Article Snippet: .. RIG-I and MDA5 were subcloned into the pQ-PGK-XIP backbone using SbfI and NotI restriction sites. pEFTak-FLAG-MAVS was a gift from Dr. Nandan Gokhale, and MAVS was subcloned into the pQ-PGK-XIP backbone using SbfI and NotI sites. hTLR3-pcDNA3 was a gift from Saumen Sarkar (Addgene plasmid # 32712) and TLR3 was subcloned into pQ-PGK-XIP using SbfI and NotI restriction sites. pcDNA3-TRIF-CFP was a gift from Doug Golenbock (Addgene plasmid # 13644) and was cloned into pQ-PGK-XIP using InFusion HD (Clonetech) pDONR223-MAP3K7 was a gift from William Hahn & David Root (Addgene plasmid # 23693) and MAP3K7 was subcloned into pQ-PGK-XIP using SbfI and NotI restriction sites. .. Point mutations in MAP3K7 were introduced using overlapping extension PCR. pcDNA4/TO/Strep-HA-ZAK alpha was a gift from Simon Bekker-Jensen (Addgene plasmid # 141193), and ZAKα was subcloned into pQ-PGK-XIP using SbfI and NotI restriction sites. pLKO-puro-CARD8 generated previously .



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